UDK: 615.013

Optimization of MTT assay for evaluation of cytotoxicity of new chemical compounds on MCF-7 cell line

D. S. Yakovlev, K. T. Sultanova, E. A. Zolotova, A. G. Gasainieva, A. A. Spasov

Волгоградский государственный медицинский университет, кафедра фармакологии и биоинформатики; Волгоградский медицинский научный центр

Abstract

It describes the features of determining of acute cytotoxicity using MTT assay. In this study the adaptation of main critical parameters of MTT assay for breast adenocarcinoma cell line has been reported. There were matched the optimal density of MCF-7 cells, time of cell adaptation and time of incubation of substances for measurement of cytotoxicity dur- ing the MTT assay.

Keywords

MTT assay, breast adenocarcinoma cell line, MCF-7

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