Volgograd Journal of Medical Research
Quarterly Journal of Science and Practice

UDK: 544.127:546.32:547.29:542.22

Characteristics of tautomeric forms of potassium orotate obtained by mechanical activation

N. N. Chuchkova, O. M. Kanunnikova, K. A. Pazinenko, M. V. Smetanina, O. V. Karban, O. L. Polyakova

Ижевская государственная медицинская академия, Россия; Ижевская государственная сельскохозяйственная академия, Россия; Первый Московский государственный медицинский университет им. И. М. Сеченова, Россия

Abstract

The purpose of the study: to obtain mechanoactivated tautomeric forms of potassium orotate, to evaluate the physical and chemical characteristics of tautomers and the effectiveness of their action on the body of laboratory animals. Re- search methods: mechanical activation of potassium orotate in a spherical planetary mill, investigation of powders by atomic force microscopy, X-ray phase analysis, NEXAFS spectrum, and investigation of the effects of the use of pow- ders of various tautomeric forms of potassium orotate on animals. It was found that the mechanical activation of the ini- tial oxo-form during 1h led to the formation of the hydroxy-form, after 6h – the dihydroxy-form, which differs in physical and chemical properties. In animal experiments, a higher efficiency of mechanoactivated drugs (hydroxy-form > dihy- droxy-form) were found to be more effective than the original oxo-form. The obtained data can be used as a basis for clinical testing of the hydroxyl-form of orotic acid, as the most active form of the drug.

Keywords

mechanical activation, potassium orotate, orotic acid tautomers, hepatopathy

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