UDK: 615.076.9:615.015.4

Using cultured hepatocytes for studying drug metabolism in human liver

K. J. Tutaev, I. S. Korotkova, A. M. Dotsenko, A. V. Strygin

ВМНЦ, лаборатория геномных и протеомных исследований; НИИ фармакологии ВолгГМУ, лаборатория психофармакологии; ВолгГМУ, кафедра фундаментальной медицины и биологии

Abstract

Due to low efficacy of methods of testing toxicity of potential drugs on preclinical stages only few drugs which were under development in laboratories go on the market. We suggested a new method of investigating drug metabolism on cultured rat and human hepatocytes with further calculating the «dose–effect» difference. These findings can be extrapo- lated to humans with the help of multiple-level computer models of drug metabolism in human’s body. This method will help to improve efficacy of preclinical studies of pharmacokinetics, interactions and toxicity of new potential medicines.

Keywords

preclinical studies, hepatocytes, cytochrome P450, drug metabolism, toxicity

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